CHEE 229

Cell-Based Engineering Principles



Laura WellsBioSci



Course Description

Introduction to the Biological, Biochemical and Life Science principles of cell/enzyme based engineering systems and processes. The emphasis will be placed on microbial cell culture, but comparisons will be drawn to related systems including viral, plant and animal cell culture as it relates to medicine, industrial practice or the environment. (0/35/0/13/0)

PREREQUISITES:  APSC 131 and APSC 132; or equivalents or permission of the Department.


Objectives and Outcomes

The objective of this course is to develop the application of the knowledge of the metabolic and molecular nature of prokaryotes and eukaryotes to solve problems in medicine, industry and the environment.  

Specific outcomes include:

  1. Identify and explain the major cellular processes in prokaryotes and eukaryotes.
  2. Describe the interrelationships between organisms and their environments.
  3. Identify and describe the relationship between structure and function on a molecular, cellular, and organismal level.
  4. Identify a range of fields where biological systems are being applied to solve engineering problems, and discuss the most recent advances in each field, as well as the strengths and limitations of each approach.
  5. Explain a variety of advanced molecular and cellular biology techniques used for the characterization and manipulation of micro-organisms, with applications in medicine, industry, and the environment.
  6. Demonstrate laboratory skills and expertise with microbiological techniques.

Specific course learning outcomes include:

CLO1 Identify the knowledge/skills required, evaluate available literature, and design experiments and/or develop methodology to meet the project objective(s) safely and efficiently. KB Bio (a)
CLO2 Apply appropriate engineering techniques, tools, and processes to accomplish the task. KB Bio (a)
CLO3 Synthesize information from experimentation, investigation, and literature to reach substantiated conclusions. KB Bio (a)
CLO4 Describe nature and possible causes of uncertainty in analysis, interpretation, and measurement. KB Bio (a)
CLO5 Generate a traceable and defensible record of the project using an appropriate project records system. KB Bio (a)
CLO6 Write and revise technical memos and reports to communicate intentions and findings using appropriate conventions and concise, precise, and clear language. IN (c)

This course develops the following attributes at the 2nd year level:

Knowledge base (KB):  Bio (a)
Applies knowledge of cellular processes to engineering problems.

Investigation (IN): (a) Design and conduct investigations to test hypotheses, related to complex problems. (c) Synthesize information from investigation, considering sources of uncertainty and limitations to reach substantiated conclusions. (c) Synthesize information from investigation, considering sources of uncertainty and limitations to reach substantiated conclusions.

Relevance to the Program

This engineering course covers cell based knowledge and principles important in biochemical and biomedical engineering. The knowledge and skills in this course will prepare students for upper year biochemical and biomedical engineering courses, such as CHEE 342, 340, 440, 484.

Course Structure and Activities

3 lecture hours per week, 1 tutorial hour in alternative weeks and three lab hours.  Times and locations can be found in SOLUS.


Required Textbook: J. Willey, L. Sherwood, C. Woolverton. 2017. Prescott, Harley and Klein’s Microbiology. 10th Edition. McGraw-Hill, Boston, MA. Print ISBN 9781259281594
(available for purchase at the bookstore, approx. $99.95)

All course lecture slides, labs, and tutorials will be posted on the CHEE 229 onQ website.