Andrew Daugulis

Queen's Research Chair in Biochemical and Cell Culture Engineering. Fellow of the Canadian Academy of Engineering. Recipient, Professional Engineers of Ontario Medal for Research and Development.

Tel: (613) 533-2784
Fax: (613) 533-6637
Email: daugulis@queensu.ca
Office: Dupuis Hall 307

Main
Publications

Research Interests

Two-Phase Partitioning Bioreactors (TPPBs)
TPPBs for Production of Neutraceuticals, Chiral Compounds and Biofuels
Treatment of Xenobiotic Compounds (gas/liquid/solid) in Partitioning Bioreactors
Bioprocess Development
Modelling and Simulation of Bioprocesses

A number of research projects are currently under investigation in my laboratory in the areas of Biochemical and Cell Culture Engineering, focused on developing novel bioprocesses for both environmental and biotechnology applications. The research from our laboratory has created a Technology Platform known as two-phase partitioning bioreactors (TPPBs), which has now been adopted by Research Groups in more than 20 countries around the world, and has led to US/Canadian patents and commercial licenses.

Since the performance of many bioprocesses is limited by the presence of toxic molecules, our strategy has been to introduce an immiscible second phase into a bioreactor, whose function is to selectively partition toxic substrates either to the microorganisms in degradative reactions, or toxic products away from the microorganisms in synthesis reactions (hence "TPPB"). In this way the microenvironment of cells is effectively detoxified of inhibitory substrates and/or products, resulting in significantly enhanced cell, and therefore process, performance. Successful examples have included the production of (inhibitory) biofuels such as ethanol and acetone/butanol, and the biodestruction of toxic substrates such as substituted phenols, endocrine disruptors, and PCBs. Environmental TPPB projects currently underway include the treatment of recalcitrant xenobiotics such as are present in fracking fluids, while Biotechnology TPPB applications include the biosynthetic production of commercially important high value neutraceuticals and biopharmaceutical precursor molecules. Through the application of rigorous thermodynamic predictions and experimental validation, the active sequestering phases in TPPBs in our laboratory now include immiscible organic solvents, ionic liquids, amorphous polymers, and ionomeric materials all acting to detoxify, and greatly enhance, bioprocess performance.

Editorial Board Activities

Editor, Journal of Hazardous Materials

Advisory Editorial Board, Journal of Chemical Technology and Biotechnology

Editorial Board, Biodegradation

Editorial Board, Biotechnology Letters

Editorial Board, Environmental Engineering and Management Journal

Funding

NSERC (Discovery, Strategic and CRD Grants), Scientific Applications International Corporation (SAIC Canada), E.I. DuPont de Nemours and Company (Wilmington DE), DuPont (Canada), Kingston Process Metallurgy

Recent Representative Publications

Journal Publications

Vincent, R. H., Parent, J.S. and Daugulis, A.J, Using poly(vinyldodecylimidazolium bromide) for the in-situ product recovery of n-butanol, Biotechnol. Prog., 2020 (In Press)

Mullins, N.R and Daugulis, A.J., The Biological Treatment of Synthetic Fracking Fluid in an Extractive Membrane Bioreactor: Selective Transport And Biodegradation Of Hydrophobic And Hydrophilic Contaminants, J.Hazard. Materials, 371, 734-742, (2019).

Mullins, N.R and Daugulis, A.J., Characterization of Transport Through Polymers for Fracking Fluid Treatment and Organic Acid Concentration in Extractive Membrane Bioreactors, J.Chem Technol Biotechnol, 94, 690-700, (2019).

Angelucci, D.M., Annesini, M.C., Daugulis, A.J. and Tomei, M.C., Polymer Extraction and Ex SituBiodegradation of Xenobiotic Contaminated Soil: Modeling of the Process Concept, J. Env. Mgmt, 230, 63-74 (2019).

Priebe, X. And Daugulis, A.J., Thermodynamic Affinity-Based Considerations for the Rational Selection of Biphasic Systems for Microbial Flavour and Fragrance Production, J.Chem.Technol.Biotechnol.,93, 656-666 (2018) (More Information).

Bacon, S.L., Ross, R.J., Daugulis, A.J. and Parent, J.S., Imidazolium-based Polyionic Liquid Absorbents for Bioproduct Recovery, Green Chemistry, 19, 5203-5213, (2017) (More Information).

Tomei, M.C., Angelucci, D.M., Stazi, V. and Daugulis, A.J. On The Applicability of a Hybrid Bioreactor Operated With Polymeric Tubing for the Biological Treatment of Saline Wastewater, Science of the Total Environment, 599-600, 1056-1063, (2017) (More Information).

Angelucci, D.M., Stazi, V., Daugulis, A.J., and Tomei, M.C., Treatment of Synthetic Tannery Wastewater in a Continuous Two-Phase Partitioning Bioreactor: Biodegradation of the Organic Fraction and Chromium Separation, J Cleaner Production, 152, 321-329 (2017) (More Information).

Tomei, M.C., Mosca Angelucci, D. and Daugulis, A.J., A Novel Continuous Two-Phase Partitioning Bioreactor Operated With Polymeric Tubing: Performance Validation for Enhanced Biological Removal of Toxic Substrates, J. Env. Management, 187, 265-272 (2016). (More Information)

Bacon, S.L., Daugulis, A.J. and Parent, J.S., Isobutylene-rich imidazolium ionomers for use in two-phase partitioning bioreactors, Green Chemistry, 18, 6586-6595 (2016). (More Information)

Tomei, M.C., Angelucci, D.M and Daugulis, A.J., Sequential Anaerobic-aerobic Decolourization of a Real Textile Wastewater in a Two-Phase Partitioning Bioreactor, Science of the Total Environment, 573, 585-593 (2016). (More Information)

Kleczek, M.R., Whitney, R.A., Daugulis, A.J. and Parent, J.S., Synthesis and Characterization of Thermoset Imidazolium Bromide Ionomers, Reactive and Functional Polymers, 106, 69-75, (2016) (More Information).

Tomei, M.C., Angelucci, D.M. and Daugulis, A.J., Towards a Continuous Two-Phase Partitioning Bioreactor for Xenobiotic Removal, J. Haz Mat., 317, 403-415, (2016). (More Information).

Ma, X-k, Ding, N., Peterson, E.C., and Daugulis, A.J. Heavy Metals Species Affect Fungal-Bacterial Synergism During the Bioremediation of Fluoranthene, Appl. Microbiol. Biotechnol., 100, 7741-7750, (2016) (More Information).

Bacon, S.L., Daugulis, A.J. and Parent, J.S., Effect of Polymer Molecular Weight Distribution on Solute Sequestration in Two-Phase Partitioning Bioreactors, Chem Eng J., 299, 56-62, (2016) (More Information).

Annesini, M.C., Tomei, M.C., Piemonte, V. and Daugulis, A.J. Xenobiotic Removal from Wastewater in a Two-phase Partitioning Bioreactor: Process modelling and Identification of Operational Strategies, Chem Eng J, 296, 428-436, (2016) (More Information).

Peterson, E.C., Contreras-López, D., Harris, J., Parent, J.S. and Daugulis, A.J., Characterization of pH Dependence in Organic Acid Absorption with Non-reactive and Reactive Polymers for Application in Two-Phase Partitioning Bioreactors, Chem. Eng J., 287, 503-510, (2016). [More Information]

Bacon, S.J., Peterson, E.C., Daugulis, A.J. and Parent J.S., Selecting Polymers for Two-Phase Partitioning Bioreactors (TPPBs): Consideration of Thermodynamic Affinity, Crystallinity and Glass Transition Temperature, Biotechnol. Prog., 31, 1500-1507, (2015). [More Information]

Harris, J. and Daugulis, A.J., Biocompatibility of Low Molecular Weight Polymers for Two-Phase Partitoning Bioreactors, Biotechnol. Bioeng., 112, 2450-2458, (2015). [More Information]

Pittman, M.J., Bodley, M.W. and Daugulis, A.J., Mass Transfer Considerations in Solid-Liquid Two-Phase Partitioning Bioreactors: A Polymer Selection Guide, J. Chem Technol. Biotechnol., 90, 1391-1399, (2015). [More Information]

Tomei, M.C., Angelucci, D.M., Ademollo, N., and Daugulis, A.J., "Rapid and Effective Decontamination of Chlorophenol-Contaminated Soil by Sorption into Commercial Polymers: Concept Demonstration and Process Modeling", Journal of Environmental Management, 150, 81-91, 2015. [More Information]

Peterson, E.C. and Daugulis, A.J., "The Use Of High Pressure CO2-Facilitated pH Swings to Enhance In Situ Product Recovery of Butyric Acid in a Two-Phase Partitioning Bioreactor," Bioitechnol. Bioeng. 111, 2183-2191, 2014. [More Information]

Dafoe, J.T. Parent, J.T and Daugulis, A.J., "Block Copolymers as Sequestering Phases in Two-Phase Biotransformations: Effect of Constituent Homopolymer Properties on Solute Affinity," J. Chem. Technol. Biotechnol. 89, 1304-1310, 2014. [More Information]

Bacon, S.L., Parent, J.S. and Daugulis, A.J., "A Framework to Predict and Experimentally Evaluate Polymer-Solute Thermodynamic Affinity for Two-Phase Partitioning Bioreactor (TPPB) Applications," J. Chem Technol. Biotechnol. 89, 948-956, 2014 [More information].

Craig, Y. and Daugulis, A.J., "Strategies for Improved Bioproduction of Benzaldehyde by Pichia pastoris and the Use of Hytrel as Tubing Material for Integrated Product Removal by in situ Pervaporation," Biochem. Eng. Journal 82, 97-104, 2014 [More information].

Ma, X.K. and Daugulis, A.J., "Transformation of Ferulic Acid to Vanillin Using a Fed-Batch Solid–Liquid Two-Phase Partitioning Bioreactor," Biotechnol. Progr. 30, 207-214, 2014 [More information].

Dafoe, J.T. and Daugulis, A.J., "In-situ Product Removal in Fermentation Systems: Improved Process Performance and Rational Extractant Selection," Biotechnol Letters 36, 443-460, 2014 [More information].

Ma, X. K. and Daugulis, A.J., "Effect of Bioconversion Conditions on Vanillin Production by Amycolatopsis sp. ATCC 39116 Through an Analysis of Competing By-product Formation," Bioproc Biosys Eng 37, 891-899, 2014 [More information].

Annesini, M.C., Piemonte, V., Tomei, M.C. and Daugulis, A.J., "Analysis of the performance and criteria for rational design of a Sequencing Batch Reactor for Xenobiotic Removal," Chem Eng Journal 235, 167-175, 2014 [More information].

Peterson, E.C. and Daugulis, A.J., "Demonstration of in situ Product Recovery of Butyric Acid via CO2-Facilitated pH Swings and Medium Development in Two-Phase Partitioning Bioreactors," Biotechnol. Bioeng. 111, 537-544, 2014 [More information].

Dafoe, J.T., and Daugulis, A.J., "Production of 4-valerolactone by an Equilibrium-Limited Transformation in a Partitioning Bioreactor: Impact of Absorptive Polymer Properties," Bioprocess and Biosystems Engineering 37, 533-542, 2014 [More information].

Tomei, M.C., Angelucci, D.M., and Daugulis, A.J., "The Use of Used Automobile Tires in a Partitioning Bioreactor for the Biodegradation of Xenobiotic Mixtures," Environmental Technology 35, 75-81, 2014 [More information].

Poleo, E.E. and Daugulis, A.J., "A Comparison of Three First Principles’ Methods for Predicting Solute-Polymer Affinity, and the Simultaneous Biodegradation of Phenol and Butyl Acetate in a Two-Phase Partitioning Bioreactor," Journal of Chemical Technology and Biotechnology 89, 88-96, 2014 [More information].

Tomei, M.C., Angelucci, D.M., Annesini, M.C. and Daugulis, A.J., "Ex situ Remediation of Polluted Soils by Absorptive Polymers, and a Comparison of Slurry and Two-Phase Partitioning Bioreactors for Ultimate Contaminant Degradation," Journal of Hazardous Materials 262, 31-37, 2013 [More information].

Dafoe, J.T., and Daugulis, A.J., "Manipulating the Composition of Absorbent Polymers Affects Product and By-product Concentration Profiles in the Biphasic Biotransformation of Indene to cis-1,2-indandiol," Biochemical Engineering Journal 77, 7-14, 2013 [More information].

Tomei, M.C. and Daugulis, A.J., "Feasibility of operating a solid-liquid bioreactor with used automobile tires as the sequestering phase for the biodegradation of inhibitory compounds," Journal of Environmental Management 125, 7-11, 2013 [More information].

Poleo, E.E and Daugulis, A.J., "Simultaneous Biodegradation of Volatile and Toxic Contaminant Mixtures by Solid-Liquid Two-Phase Partitioning Bioreactors," J Hazardous Materials 254-255, 206-213, 2013 [More information].

Yun, J. Dafoe, J.T., Peterson, E.C., Xuc, L., Yao, S.J. and Daugulis, A.J., "Rapid Freezing Cryo-Polymerization and Microchannel Liquid-Flow Focusing for Cryogel Beads: Adsorbent Preparation and Characterization of Supermacroporous Bead-Packed Beds,"Journal of Chromatography A 1284, 148-154, 2013 [More information].

Craig, T. and Daugulis, A.J., "Polymer Characterization And Optimization Of Conditions For The Enhanced Bioproduction of benzaldehyde by Pichia pastoris in a Two-Phase Partitioning Bioreactor," Biotechnol. Bioeng. 110, 1098-1105, 2013 [More information].

Tomei, M.C. and Daugulis. A.J., "Ex-situ Bioremediation of Contaminated soils: An Overview of Conventional and Innovative Technologies," Crit Rev Environ Sci Technol 43, 2107-2139, 2013 [More information].

Munoz, R., Daugulis, A.J., Hernandez, M. and Quijano, G., "Recent Advances in Two-Phase Partitioning Bioreactors for the Treatment of Volatile Organic Compounds," Biotechnology Advances 30, 1707-1720, 2012 [More information].

Daugulis, A.J. and Milton S.G., "Two-phase Partitioning Bioreactors: The Use of Polymers for the in situ Removal of Ethanol," Asia-Pacific Journal of Chemical Engineering 7, 324-328, 2012 [More information].

Tomei, M.C., Annesini, M.C. and Daugulis, A.J., "2,4-Dichlorophenol Removal In A Solid-Liquid Two Phase Partitioning Bioreactor (TPPB): Kinetics Of Absorption, Desorption And Biodegradation," New Biotechnol. 30, 44-50, 2012 [More information].

Tomei, M.C., Annesini and Daugulis, A.J., "Solid-Liquid Two-Phase Partitioning Bioreactors (TPPBs) Operated with Waste Polymers. Case Study: 2,4 Dichlorophenol Biodegradation with Used Automobile Tires as the Partitioning Phase," Biotechnol. Letters 34, 2037-2042, 2012 [More information].

Hagesteijn, K.F.L. and Daugulis, A.J., "Passive/Aggressive Detoxification of Continuous Flow Biotreatment Systems Using Absorptive Polymers: Partitioning Bioreactors Treating Transient Phenol Loadings," Biotechnol Letters 34, 1817-1824, 2012 [More information].

Fam H., and Daugulis, A.J., "Substrate Mass Transport in Two-Phase Partitioning Bioreactors Employing Liquid and Solid Non-Aqueous Phases," Bioproc. Biosystem. Eng. 35, 1367-1374, 2012 [More information].

Parent, J.S., Capela, M., Dafoe, J.T. and Daugulis, A.J., "First Principles Approach to Identifying Polymers for Use in Two-Phase Partitioning Bioreactors," J Chem Technol Biotechnol 87, 1059-1065, 2012 [More information].

Hepburn, A.J. and Daugulis, A.J., , "The Use of CO2 for Reversible pH Shifting, and the Removal of Succinic Acid in a Polymer-Based Two-Phase Partitioning Bioreactor," J Chem Technol Biotechnol 87, 42-50, 2012 [More information].

Dafoe, J., and Daugulis, A.J., "Bioproduction of cis-(1S,2R)-indandiol, a Chiral Pharmaceutical Intermediate, Using a Solid-Liquid Two-Phase Partitioning Bioreactor (TPPB) for Enhanced Removal of Inhibitors," J. Chem Technol. Biotechnol. 86, 1379-1385, 2011 [More information].

Tomei, M.C., Rita, S., Angelucci, D.M., Annesini, M.C. and Daugulis, A.J., "Treatment of Substituted Phenol Mixtures in Single Phase and Two-Phase Solid-Liquid Partitioning Bioreactors," J. Hazardous Materials 191, 190-195, 2011 [More information].

Daugulis, A.J., Tomei, M.C. and Guieysse, B. , "Overcoming Substrate Inhibition During Biological Treatment of Mono-Aromatics: Recent Advances in Bioprocess Design. ," Appl. Microbiol. Biotechnol. 90, 1589-1608, 2011 [More information].

Montes, M., Daugulis, A.J., Veiga, M.C. and Kennes, C., "Characterization of Absorbent Polymers for the Removal of Volatile Hydrophobic Pollutants from Air," J. Chem Technol. Biotechnol. 86, 47-53, 2011 [More information].

Khan, T.R. and Daugulis, A.J., "The Effects of Polymer Phase Ratio and Feeding Strategy on Solid-Liquid TPPBs for the Production of L-phenylacetylcarbinol from Benzaldehyde Using Candida utilis," Biotechnol. Letters 33, 63-70, 2011 [More information].

Khan, T.R. and Daugulis, A.J., "Composition Effects on Solute Partitioning in Solid-Liquid Two-Phase Bioreactors,," J. Chem. Technol. Biotechnol. 86, 157-160, 2011 [More information].

Hernandez, M., Munoz, R. and Daugulis, A.J., "Biodegradation of VOC Mixtures of Different Hydrophobicities in Two-Phase Partitioning Bioreactors Containing Tailored Polymer Mixtures," J Chem Technol Biotechnol 86, 138-144, 2011 [More information].

Jain, A.N., Khan, T.R. and Daugulis, A.J., "Bioproduction of Benzaldehyde in a Solid-Liquid Two-Phase Partitioning Bioreactor using Pichia pastoris," Biotechnol. Letters 32, 1649-1654, 2010 [More information].

Yeom, S.H. and Daugulis, A.J. and Lee, S.H., "Bioremediation of Phenol-Contaminated Water and Soil Using Magnetic Polymer Beads," Process Biochem. 45, 1582-1586, 2010 [More information].

Yeom, S.H., Daugulis, A.J. and Nielsen, D.R., "A Strategic Approach for the Design and Operation of Two-phase Partitioning Bioscrubbers for the Treatment of Volatile Organic Compounds," Biotechnol. Progr. 26, 1777-1786, 2010 [More information].

Khan, T.R. and Daugulis, A.J., "Application of Solid-Liquid TPPBs to the Production of L-Phenylacetylcarbinol from Benzaldehyde Using Candida utilis," Biotechnol. Bioeng. 107, 4, 633-641, 2010 [More information].

Tomei., M.C., Annesini, M.C., Rita, S. and Daugulis, A.J., "Two Phase Partitioning Bioreactors Operating with Polymers Applied to the Removal of Substituted Phenols," Env. Sci. & Technol. 44, 7254-7259, 2010 [More information].

Hernandez, M., Quijano, G., Thalasso, F., Daugulis, A.J., Villaverde, S. and Munoz, R., "A Comparative Study of Solid and Liquid Non-Aqueous Phases for the Biodegradation of Hexane in Two-Phase Partitioning Bioreactors," Biotechnol. Bioeng. 106, 731-740, 2010 [More information].

Tomei, M.C., Annesini, M.C., Piemonte, V., Prpich, G.P. and Daugulis, A.J., "Two Phase Reactors Applied to the Removal of Substituted Phenols: Comparison Between Liquid-Liquid and Liquid-Solid Systems," Water Science and Technology 62, 776-782, 2010 [More information].

Isaza, P.A., Daugulis, A.J. and Kuran, K., "Mass Transport and Thermodynamic Analysis of PAHs in Partitioning Systems in the Presence and Absence of Ultrasonication," AIChE Journal 56, 10, 2717-2726, 2010 [More information].

Isaza, P.A. and Daugulis, A.J., "Enhanced Degradation of Phenanthrene in a Solid-Liquid Two-Phase Partitioning Bioreactor via Sonication," Biotechnol. Bioeng. 105, 997-1001, 2010 [More information].

Yeom, S.H., Daugulis, A.J. and Nielsen, D.R., "Estimating The Cellular Maintenance Coefficient and Its Use in the Design of Two-Phase Partitioning Bioscrubbers," Bioproc Biosys Eng 33, 731-739, 2010 [More information].

Littlejohns, J.V., McAuley, K.B. and Daugulis, A.J., "Model for a Solid-Liquid Stirred Tank Two-Phase Partitioning Bioscrubber for the Treatment of BTEX," Journal of Hazardous Materials 175, 872-882, 2010 [More information].

Gao, F. and Daugulis, A.J., "Polymer-Solute Interactions In Solid-Liquid Two-Phase Partitioning Bioreactors," J. Chem. Technol. Biotechnol. 85, 302-306, 2010 [More information].

Littlejohns, J.V., McAuley, K.B. and Daugulis, A.J., "Model for a Solid-Liquid Airlift Two-Phase Partitioning Bioscrubber for the Treatment of BTEX," J. Chem. Technol. Biotechnol. 85, 173-184, 2010 [More information].

Tomei, M.C., Annesini, M.C., Prpich, G.P. and Daugulis, A.J., "Biodegradation of 4-Nitrophenol in a Two Phase System Operating with Polymers As the Partitioning Phase," Env. Sci. & Technol. 43, 7105-7110, 2009 [More information].

Littlejohns, J.V. and Daugulis, A.J., "Oxygen Mass Transfer and Hydrodynamics in a Multi-Phase Airlift Bioscrubber System," Chem Eng. Sci. 64, 4171-4177, 2009 [More information].

Gao, F. and Daugulis, A.J., "Bioproduction of the aroma compound 2-phenylethanol in a solid-liquid Two-Phase Partitioning Bioreactor system by Kluyveromyces marxianus," Biotechnol. Bioeng. 104, 332-339, 2009 [More information].

Isaza, P.A. and Daugulis, A.J., "Ultrasonically Enhanced Delivery and Degradation of PAHs in a Polymer-Liquid Partitioning System by a Microbial Consortium," Biotechnol. Bioeng. 104, 91-101, 2009 [More information].

Littlejohns, J.V and Daugulis, A.J., "A Two-Phase Partitioning Airlift Bioreactor for the Treatment of BTEX Contaminated Gases,"Biotechnol. Bioeng. 103, 6, 1077-1086, 2009 [More information].

Littlejohns, J.V. and Daugulis, A.J., "Response of a Solid-Liquid Two-Phase Partitioning Bioreactor to Transient BTEX Loadings,"Chemosphere 73, 1453-1460, 2008 [More information].

Tomei, M.C., Annesini, M.C., Rita, S. and Daugulis, A.J., "Biodegradation of 4-nitrophenol in a Two Phase Sequencing Batch Reactor: Concept Demonstration, Kinetics and Modelling," Appl. Microbiol. Biotechnol. 80, 1105-1112, 2008 [More information].

Rehmann, L., Prpich, G.P. and Daugulis, A.J., "Bioremediation of PAH Contaminated Soils: Application of a Solid-Liquid Two-Phase Partitioning Bioreactor," Chemosphere 73, 798-804, 2008 [More information].

Littlejohns, J.V. and Daugulis, A.J., "Kinetics and Interactions of BTEX Compounds During Degradation by a Bacterial Consortium,"Process Biochem 43, 1068-1076, 2008 [More information].

Morrish, J.L.E. and Daugulis, A.J., "Improved Reactor Performance and Operability in the Biotransformation of Carveol to Carvone Using a Solid-liquid Two Phase Partitioning Bioreactor," Biotechnol. Bioeng. 101, 946-956, 2008 [More information].

Morrish, J.L.E., Brennan, E., Dry, H. and Daugulis, A.J., "Enhanced Bioproduction of Carvone in a Two Liquid Phase Partitioning Bioreactor with a Highly Hydrophobic Biocatalyst," Biotechnol. Bioeng. 101, 768-775, 2008 [More information].

Prpich, G.P., Rehmann, L. and Daugulis, A.J., "On The Use, and Re-Use, of Polymers for the Treatment of Hydrocarbon Contaminated Water Via a Solid-Liquid Partitioning Bioreactor," Biotechnol. Prog. 24, 839-844, 2008 [More information].

Daugulis, A.J. and Boudreau, N.G., "Solid-Liquid Two-Phase Partitioning Bioreactors for the Treatment of Gas-Phase VOCs by a Microbial Consortium," Biotechnol Letters 30, 1583-1587, 2008 [More information].

Daugulis, A.J., "A Survey Of Bioengineering Research In Canada-2007," Biotechnol. Prog. 24, 795-806, 2008 [More information].

Rehmann, R. and Daugulis, A.J., "Biodegradation of PCBs in Two-Phase Partitioning Bioreactors Following Solid Extraction from Soil," Biotechnol. Bioeng. 99, 1273-1280, 2008 [More information].

Morrish, J.L.E. and Daugulis, A.J., "Inhibitory effects of substrate and product on the carvone biotransformation activity of Rhodococcus erythropolis ," Biotechnol. Letters 30, 1245-1250, 2008 [More information].

McMahon, M.J.L. and Daugulis, A.J., "Enhancement of Biogenic Sulfide Production in a Packed Bed Bioreactor via Critical Inoculum Design and Carrier Material Selection," Biotechnol. Bioeng. 100, 855-863, 2008 [More information].

McMahon, M.J.L. and Daugulis, A.J., "Gas Phase H2S Product Recovery In A Packed Bed Bioreactor With Immobilized Sulfate Reducing Bacteria ," Biotechnol. Letters 30, 467-473, 2008 [More information].

Rehmann, L. and Daugulis, A.J., "Enhancement of PCB Degradation by Burkholderia xenovorans LB400 in Biphasic Systems by Manipulating Culture Conditions," Biotechnol. Bioeng. 99, 3, 521-258, 2008 [More information].

Rehmann, L. and Daugulis, A.J, "Bioavailability of PCBs in Biphasic Bioreactors," Biochem Eng J 38, 219-225, 2008 [More information].

Rehmann, L. and Daugulis, A.J., "Polymer Selection for Biphenyl Degradation in a Solid-Liquid Two-Phase Partitioning Bioreactor," Biotechnol. Prog. 23, 4, 814-819, 2007 [More information].

Prpich, G.P and Daugulis, A.J., "A Novel Solid-Liquid Two-Phase Partitioning Bioreactor for the Enhanced Bioproduction of 3-methylcatechol," Biotechnol. Bioeng. 98, 1008-1016, 2007 [More information].

Rehmann, L. and Daugulis, A.J., "Biodegradation of Biphenyl in a Solid-Liquid Two-Phase Partitioning Bioreactor," Biochem Eng J36, 195-201, 2007 [More information].

Nielsen, D.R., Daugulis, A.J. and McLellan, P.J., "Dynamic Simulation of Benzene Vapor Treatment by a Two-Phase Partitioning Bioscrubber. Part II: Model Calibration, Validation, and Predictions," Biochem Eng J 36, 250-261, 2007 [More information].

Nielsen, D.R., Daugulis, A.J. and McLellan, P.J., "Dynamic Simulation of Benzene Vapor Treatment by a Two-Phase Partitioning Bioscrubber. Part I: Model Development, Parameter Estimation, and Parametric Sensitivity," Biochem Eng J 36, 239-249, 2007[More information].

Vandermeer, K.D. and Daugulis, A.J., "Enhanced Degradation of a Mixture of Polycyclic Aromatic Hydrocarbons by a Defined Microbial Consortium in a Two-Phase Partitioning Bioreactor," Biodegradation 18, 211-221, 2007 [More information].

Littlejohns, J.V. and Daugulis, A.J., "Oxygen Transfer in a Gas-Liquid System Containing Solids of Varying Oxygen Affinity," Chem Eng J 129, 67-74, 2007 [More information].

Prpich, G.P. and Daugulis, A.J., "Solvent Selection For Enhanced Bioproduction of 3-methylcatechol in a Two-Phase Partitioning Bioreactor," Biotechnol. Bioeng. 97, 536-543, 2007 [More information].

Eibes, G., M.T. Moreira, M.T., Feijoo, G, Daugulis, A.J., and Lema, J.M., "Operation Of A Two Phase Partitioning Bioreactor for the Degradation of Anthracene by the Enzyme Manganese Peroxidase," Chemosphere 66, 1744-1751, 2007 [More information].

Prpich, G.P., Adams, R.L., and Daugulis, A.J., "Ex situ bioremediation of phenol contaminated soil using polymer beads,"Biotechnology Letters 28, 24, 2027-2031, 2006 [More information].

Nielsen, D.R., Sask, K.N., McLellan, P.J. and Daugulis, A.J., "Benzene Vapor Treatment Using a Two-Phase Partitioning Bioscrubber: An Improved Steady-State Protocol to Enhance Long-Term Operation," Bioproc. Biosyst. Eng. 29, 229-240, 2006[More information].

Nielsen, D.R., McLellan, P.J. and Daugulis, A.J., "Direct estimation of the oxygen requirements of Achromobacter xylosoxidans for aerobic degradation of monoaromatic hydrocarbons (BTEX) in a bioscrubber," Biotechnol. Letters 28, 16, 1293-1298, 2006 [More information].

Prpich, G.P and Daugulis, A.J., "Biodegradation of a Phenolic Mixture in a Solid-Liquid Two Phase Partitioning Bioreactor," Appl. Microbiol. Biotechnol. 72, 607-615, 2006 [More information].

Boudreau, N.G. and Daugulis, A.J., "Transient Performance Of Two-Phase Partitioning Bioreactors Treating A Toluene Contaminated Gas Stream," Biotechnol Bioeng. 94, 3, 448-457, 2006 [More information].

Tyshenko, M.G., d'Anjou, M., Davies, P.L., Daugulis, A.J., and Walker, V.K., "Challenges In The Expression Of Disulfide Bonded, Threonine-Rich Antifreeze Proteins In Bacteria And Yeast," Protein Expression and Purification 47, 1, 152-161, 2006 [More information].

Rehmann L., and Daugulis, A.J., "Biphenyl degradation kinetics by Burkholderia xenovorans LB400 in two-phase partitioning bioreactors," Chemosphere 63, 6, 972-979, 2006 [More information].

Nielsen, D.R., Daugulis, A.J., and McLellan, P.J., "Transient Performance of a Two-Phase Partitioning Bioscrubber Treating a Benzene-Contaminated Gas Stream," Env. Sci Technol. 39, 8971-8977, 2005 [More information].

Nielsen, D.R., Daugulis, A.J. and McLellan, P.J., "A Restructured Framework for Modeling Oxygen Transfer in Two-Phase Partitioning Bioreactors," Biotechnol. Bioeng 91, 773-777, 2005 [More information].

Nielsen, D.R., Daugulis, A.J. and McLellan, P.J., "Quantifying Maintenance Requirements from the Steady-State Operation of a Two-Phase Partitioning Bioscrubber," Biotechnol. Bioeng 90, 248-258, 2005 [More information].

Baumann, M.D., Daugulis, A.J., and Jessop, P.G., "Phosphonium Ionic Liquids for Degradation of Phenol in a Two-Phase Partitioning Bioreactor," Appl. Microbiol. Biotechnol 67, 131-137, 2005 [More information].

Prpich, G.P. and Daugulis, A.J., "Enhanced Biodegradation of Phenol By a Microbial Consortium in a Solid-Liquid Two Phase Partitioning Bioreactor," Biodegradation 16, 329-339, 2005 [More information].

MacLeod C.T. and Daugulis, A.J., "Interfacial Effects in a Two-Phase Partitioning Bioreactor: Degradation of Polycyclic Aromatic Hydrocarbons (PAHs) by Hydrophobic Mycobacterium," Process Biochem 40, 1799-1805, 2005 [More information].

Prpich, G.P and Daugulis, A.J., "Polymer Development For Enhanced Delivery Of Phenol In A Solid-Liquid Two-Phase Partitioning Bioreactor," Biotechnol. Progr 20, 1725-1732, 2004 [More information].

Davidson, C.T. and Daugulis, A.J., "Addressing Biofilter Limitations: A Two-Phase Partitioning Bioreactor Process For The Treatment Of Benzene And Toluene Contaminated Gas Streams," Biodegradation 14, 415-421, 2003 [More information].

Amsden, B.G., Bochanysz, J., and Daugulis, A.J., "Degradation of Xenobiotics in a Partitioning Bioreactor in which the Partitioning Phase is a Polymer," Biotechnol. Bioeng 84, 399-405, 2003 [More information].

Daugulis, A.J., and McCracken, C.M., "Microbial Degradation of High and Low Molecular Weight Polyaromatic Hydrocarbons in a Two-Phase Partitioning Bioreactor by Two Strains of Sphingomonas sp.," Biotechnol. Letters 25, 1421-1424, 2003 [More information].

Daugulis. A.J. and Boudreau, N.G., "Removal and Destruction of High Concentrations Of Gaseous Toluene in a Two-Phase Partitioning Bioreactor by Alcaligenes xylosoxidans," Biotechnol. Letters 25, 1441-1444, 2003 [More information].

Daugulis, A.J., Amsden, B.G., Bochanysz, J. and Kayssi, A., "Delivery of Benzene to Alcaligenes xylosoxidans by Solid Polymers in a Two-Phase Partitioning Bioreactor," Biotechnol. Letters 25, (14), 1203-1207, 2003 [More information].

Nielsen, D.R., Daugulis, A.J. and McLellan, P.J., "A Novel Method of Simulating Oxygen Mass Transfer in Two-Phase Partitioning Bioreactors," Biotechnol.Bioeng 83, 735-742, 2003 [More information].

Davidson, C.T. and Daugulis, A.J., "The Treatment of Gaseous Benzene by Two-Phase Partitioning Bioreactors: A High Performance Alternative to the Use of Biofilters," Appl.Microbiol.Biotechnol 62, 297-301, 2003 [More information].

MacLeod, C.T. and Daugulis, A.J., "Biodegradation of Polycyclic Aromatic Hydrocarbons in a Two-Phase Partitioning Bioreactor in the Presence of a Bioavailable Solvent," Appl.Microbiol.Biotechnol 62, 291-296, 2003 [More information].

Pudge, I.B., Dubois, C. and Daugulis, A.J., "The Use of Enterobacter cloacae ATCC 43560 in the Development of a Two-Phase Partitioning Bioreactor for the Destruction of Hexahydro-1,3,5-trinitro-1,3,5-s-triazine (RDX)," J. Biotechnol 100, (1), 65-75, 2003[More information].

Janikowski, T.B., Velicogna, D., Punt, M., and Daugulis, A.J., "Use of a Two-Phase Partitioning Bioreactor for Degrading Polycyclic Aromatic Hydrocarbons by a Sphingomonas sp.," Appl Microbiol Biotechnol 59, 368-376, 2002 [More information].

Vrionis, H., Kropinski, A.M.B. and Daugulis, A.J., "Enhancement Of A Two-Phase Partitioning Bioreactor System By Catalyst Modification: Demonstration Of Concept," Biotechnol. Bioeng 79, 587-594, 2002 [More information].

Daugulis, A.J. and Janikowski, T.B., "Scale-Up Performance of a Partitioning Bioreactor for the Degradation of Polyaromatic Hydrocarbons by Sphingomonas aromaticivorans," Biotechnol. Letters 24, (8), 591-594, 2002 [More information].

Vrionis, H., Kropinski, A.M.B. and Daugulis, A.J., "Strain Modification And Feed Strategies For Expanded Application Of A Two-Phase Partitioning Bioreactor To Phenol Degradation," Biotechnology Progress 18, (3), 458-464, 2002 [More information].

Vrionis, H.A., Daugulis, A.J. and Kropinski, A.M., "Identification And Characterization Of The Agmr Regulator Of Pseudomonas putida: Role In Alcohol Utilization," Appl.Microbiol.Biotechnol 58, 469-475, 2002 [More information].

Malinowski, J.J. and Daugulis, A.J., "An Effective Approach for Recovery of Methyl-substituted 1,3-Dioxane from Aqueous Media,"Separation Science and Technology 37, (11), 2659-2667, 2002 [More information].

Daugulis, A.J., "Two-Phase Partitioning Bioreactors: A New Technology Platform for Destroying Xenobiotics," Trends in Biotechnology 19, 459-464, 2001 [More information].

Yeom, S-H, Louie, J. and Daugulis, A.J., "The Use of Partitioning Bioreactors for the Treatment of High-Concentration Benzene Solutions," Can.J.Chem.Eng. 79, (5), 785-790, 2001.

Li, Z., Xiong, F., Lin, Q., d'Anjou, M., Daugulis, A.J., Yang, D.S.C., and Hew, C.L., "Low Temperature Increases the Yield of Biologically Active Herring Antifreeze Protein in Pichia pastoris," Protein Expression and Purification 21, 438-445, 2001 [More information].

Yeom, S-H, and Daugulis, A.J., "A Two-Phase Partitioning Bioreactor System for Treating Benzene-Contaminated Soil," Biotechnol Letters 23, 467-473, 2001 [More information].

Yeom, S.H, and Daugulis, A.J., "Benzene Degradation in a Two-Phase Partitioning Bioreactor by Alcaligenes xylosoxidans Y234,"Process Biochemistry 36, 765-772, 2001 [More information].

Yeom, S.H. and Daugulis, A.J., "Development of a Novel Bioreactor System for the Treatment of Gaseous Benzene," Biotechnol. Bioeng. 72, 156-165, 2001 [More information].

d'Anjou, M.C., and Daugulis, A.J., "A Rational Approach to Improving Productivity in Recombinant Pichia pastoris Fermentation,"Biotechnol.Bioeng. 72, 1-11, 2001 [More information].

Patents

Daugulis, A.J. and Collins, D.L., "Process for Biodegradation of a Xenobiotic," US Patent No. 6,284,523, September, 2001.